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Notices
1. Shortlisting of Applicants for Master of Applied Science and PhD Programs Second Call
2. Shortlisting of Applicants for Bachelor of Science in Computer Science and Technology (BScCST) Second Call
3. The Procurement of Supply, Delivery, Installation, Testing and Commissioning of Generator with ATS Panel
4. The Procurement of Supply, Delivery, Installation, Testing and Commissioning of IT Equipment
5. Tender Notice
6. Call for Applications for Distinguished Professor Position
7. Invitation of Applications (Second Call) for Admission to Master of Applied Science and PhD Programs in Organic Agriculture | Forest Biomaterials Science and Engineering | Artificial Intelligence | Data Science | Sustainable and Resilient Infrastructure

Genomics and Tissue Culture (GATC) Lab

MBUST LABORATORY

Genomics and Tissue Culture (GATC) Lab

Cutting-edge molecular research to improve crop varieties and enhance agricultural productivity through biotechnology.

  • Plant tissue culture
  • Genome Editing & Genetic Transformation
  • Molecular Biotechnology
  • Polymerase Production
  • Greenhouse design
LEADERSHIP

Laboratory In-charge

Dr. Sarbesh Das Dangol

Dr. Sarbesh Das Dangol, Asst. Professor

Organic Agriculture Program

Email: sarbesh.das.dangol@mbust.edu.np

INFRASTRUCTURE

Major Instruments

Dr. Sarbesh Das Dangol
Horizontal laminar air flow cabinet
Dr. Sarbesh Das Dangol
Gel documentation system
Dr. Sarbesh Das Dangol
Plant growth chamber
Dr. Sarbesh Das Dangol
Electroporator
Dr. Sarbesh Das Dangol
Laboratory setup at GATC Laboratory
  • Plant Tissue Culture Instrumentation: Laminar flow cabinets (Biobase), Plant growth chamber, weighing balances, pH meter, and autoclave machines (SLEFA)
  • Molecular Biology Instruments: PCR machine (BioRad), nanodrop, gel documentation system (BioRad), SDS-PAGE system (BioRad), gel electrophoresis system, UV transilluminator, electroporator (BioRad), and Real-time PCR (Under procurement)
  • SPAD meter
  • Cooling centrifuge and Incubators
  • 4 C and -20 C refrigeration systems
  • Ice-maker and double distillation unit
  • MinION Mk1D sequencing system- UNESCO-TWAS grant (Under procurement)
  • Fluorescence microscope- UNESCO-TWAS grant (Under procurement)
RESEARCH OUTPUT

Completed Research in GATC Laboratory

Conservation of Indigenous and Nutrient-Rich Crops of Nepal and blast detection methods

Principal Investigator: Dr. Sarbesh Das Dangol,

Researchers: Dr. Jarina Joshi, Dr. Hira Kaji Manandhar, Dr. Anupama Shrestha, Ms. Samita Gharti Magar, Ms. Shruti Subedi, Ms. Dibya Chhatkuli, Mr. Pawan Kathayat, and Mr. Birendra Mahara

Completion date: July 2025

Funded by: Ministry of Education, Science and Technology (MoEST), Government of Nepal.

Small-scale production of probiotic kefir with different flavors (Co-PI)

Principal Investigator: Dr. Jarina Joshi

Researchers: Dr. Sarbesh Das Dangol, Ms. Sushmita Soni, Ms. Saru Maharjan

Completion date: July 2025

Funded by: Ministry of Education, Science and Technology (MoEST), Government of Nepal.

In vitro production and purification of polymerases

Principal Investigator: Dr. Sarbesh Das Dangol

Researchers: Ms. Alina Shri Sapkota, Dr. Gorkha Raj Giri, Dr. Priti Regmi, Ms. Manita Tamang

Completion date: June 2026

Funded by: Nepal Academy of Science and Technology

Students Involved in Research

Arbind Singh Kushwaha

Greenhouse design (arbind.singh.kushwaha@mbust.edu.np)

Birendra Mahara

Blast pathogen isolation and characterization (birendra.mahara@mbust.edu.np)

Manita Tamang

Polymerase production and purification (manita.tamang@mbust.edu.np)

Naresh Bahadur Deuba

Greenhouse design (naresh.bahadur.deuba@mbust.edu.np)

Pawan Kathayat

Genome editing in rice (pawan.kathayat@mbust.edu.np)

Prakash Kumal

Millet genetic engineering (prakash.kumal@mbust.edu.np)

Pratistha Maharjan

Potato microtuber research and tissue culture (pratistha.maharjan@mbust.edu.np)

Roshan Dhungana

Millet genetic engineering (roshan.dhungana@mbust.edu.np)

Roshani Dhami

Tea micropropagation (roshani.dhami@mbust.edu.np)

Our Collaborators

  • Central Department of Biotechnology, Tribhuvan University

  • Jun Chiyabari Tea Garden Pvt. Ltd.

  • Pragatishil Yuva Krishi Krishak Samuha

  • Nepal Plant Disease and Agro Associates Pvt. Ltd.

  • Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Pakistan

  • Nepal Academy of Science and Technology

  • Nepal Agricultural Research Council

Social Media Handle

@GATCLab on Instagram and X.